Unlocking the core characteristics of cell lines and laying a solid foundation for industrialization
Next-generation sequencing (NGS) technology offers comprehensive cell line analysis solutions with its deep coverage capabilities , enabling systematic research on the genetic characteristics, identity markers, and safety background of various cell lines. This provides precise data support for cell line screening, validation, application, and quality control.
Whole - genome NGS sequencing
By employing high-depth whole-genome sequencing technology, combined with an advanced bioinformatics analysis platform, we can achieve a comprehensive and precise understanding of cell lines:
High-depth coverage
Sequencing depth can reach ≥50×, ensuring the capture of key information such as low-abundance gene variants and inserted sequences;
Multi-dimensional analysis
Integrating multi-level data such as genome and transcriptome, providing a comprehensive analysis from the dimensions of genetic characteristics, functional properties, and safety risks;
Standardized processes
Strictly adhering to laboratory quality control standards, the data analysis process is traceable and repeatable, ensuring the accuracy and reliability of the results;
Testing Content
• Core function: By detecting cell line-specific short tandem repeat (STR) sequences and comparing them with international standard databases (such as ATCC and DSMZ), the authenticity of the cell line identity can be confirmed;
• Detection advantages: Accurately distinguishing the origin of cell lines and avoiding cross-contamination (such as confusion between human and mouse cell lines) is the core guarantee for data reliability and industrial compliance ;
Core functions: Accurately detect the insertion site, copy number, and sequence integrity of the target gene in genetically engineered cell lines (such as recombinant protein expression cell lines and gene editing cell lines);
• Gene mutation detection: Comprehensive screening of genetic abnormalities such as gene mutations (SNV), copy number variations (CNV), and structural variations (SV) in cell lines , and analysis of the association between cell line phenotype and genetic characteristics;
• Chromosomal integrity analysis: Detects structural variations such as abnormal chromosome number, translocation, and deletion, and assesses the genetic stability of cell lines;
• Functional gene analysis: Analyze the expression characteristics and regulatory mechanisms of genes related to core cell line functions (such as proliferation, differentiation, and metabolism genes) to provide direction for cell line optimization.
Types of cell lines covered
Engineered cell lines
recombinant protein expression cell lines (CHO, HEK293), gene editing cell lines (CRISPR/Cas9 modified cells), CAR engineered cell lines, etc.;
Stem cell lines
Analysis of genetic stability and differentiation potential of embryonic stem cells (ESC), induced pluripotent stem cells (iPSC), mesenchymal stem cells (MSC);
Routine cell lines
Identification, contamination screening, and genetic characteristic verification of routine experimental cell lines used in scientific research (such as HeLa, A549, MCF-7).
Application Scenarios
Basic research stage
cell line authentication, genetic characteristic analysis, and experimental protocol optimization to ensure the authenticity and reproducibility of data;
Biopharmaceutical R&D
Recombinant cell line construction and screening, target gene insertion verification, and cell line stability monitoring, contributing to the development of protein drugs and antibody drugs;
In the field of cell therapy
assessing the genetic stability of stem cell lines and engineered cell lines, identifying safety risks, and providing compliance support for clinical applications;
Quality control phase
stability monitoring, identity consistency verification, and contamination investigation during cell line passaging to ensure compliance of the production process .