Key Design Points of Four Methods
Identify detection targets such as viral antigens and specific proteins.
Optimize the reaction system and set the linear range and minimum detection limit.
Design specific primers/probes to avoid cross-reactions
Adjusting the reaction program, including annealing temperature and extension time.
Screening internal reference genes to ensure the accuracy of data normalization
Screening for highly specific antibody pairs (capture + detection of antibodies)
Optimize coating concentration, blocking solution, and incubation time parameters.
Select suitable host cells and determine the inoculation density and culture period.
Establish interpretation criteria such as fluorescence intensity threshold and cytopathic effect score.
Optimize Process
By employing single-factor variable methods or orthogonal experiments, key parameters such as reaction temperature and reagent dosage are iteratively tested. Through closed-loop optimization of "parameter adjustment - result verification - deviation analysis", the accuracy, precision and robustness of the method are improved.
Core Advantages
Covering four core detection types and adaptable to various biological samples
Customized solutions to meet different testing accuracy requirements
Closed-loop optimization process ensures the stability and reliability of the method.