Key Design Considerations for Four Methods
Bioassay
Identify detection targets such as viral antigens and specific proteins.
Optimize the reaction system; set targets for linear range and limit of detection (LOD).
ELISA (Enzyme-Linked Immunosorbent Assay)
Screen highly specific antibody pairs (capture and detection antibodies).
Optimize coating concentration, blocking buffer, and incubation time parameters.
qPCR (Real-Time Quantitative PCR)
Design specific primers/probes to avoid cross-reactivity.
Adjust reaction parameters including annealing temperature and extension time.
Select reference genes to ensure accurate data normalization.
Cell-Based Assay
Select appropriate host cells; determine seeding density and culture duration.
Establish interpretation criteria, such as fluorescence intensity thresholds and cytopathic effect (CPE) scoring.
Optimization Workflow
Use single-factor or orthogonal experiments to iteratively test key parameters including reaction temperature and reagent volumes. Through a closed-loop optimization process of "parameter adjustment → result verification → deviation analysis," improve the accuracy, precision, and robustness of the method.
Core Advantages
NO.1
Covers four core detection types, compatible with various biological samples.
NO.2
Customizable solutions tailored to different detection accuracy requirements.
NO.3
Closed-loop optimization process ensures method stability and reliability.