Pichia ResDNA Quantitation Kit [HYR004]

Type:qPCR Detection
Product Code:HYR004
Specification:100 tests/kit
Quantification Range:30 fg/μL–300 pg/μL

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Product Introduction

Product Overview

The Pichia resDNA Quantitation Kit employs the TaqMan fluorescent probe qPCR method, utilised in conjunction with the Host Cell Residual DNA Sample Nucleic Acids Extraction Kit, to accurately, efficiently, and specifically quantify Pichia pastoris host cell DNA in intermediates, semi-finished products, and finished products of various biological products.

Product Performance

Validation Parameters		Validation Results
Linearity Range	Quantification Range	30 fg/μL–300 pg/μL
	R²	≥0.98
	Amplification efficiency	90%–110%
Accuracy	Deviation from Enterprise Reference DNA	CV ≤ 5%
Precision	Intermediate precision	CV ≤ 15%
Precision	Repeatability	CV ≤ 15%
Recovery rate	Simulated sample recovery rate	70%-130%
Specificity		Specific detection of residual DNA in Pichia pastoris cells, unaffected by interference from exogenous cells such as CHO, HEK293, or Vero
Stability		1) Performance unchanged after five freeze-thaw cycles2) Performance unchanged after storage at 4°C for over one month

Product Documentation Download

Related products

Reagent kit name	Product Code
Host Cell Residual DNA Sample Nucleic Acids Extraction Kit (Magnetic Bead Method)	HYS001
Host Cell Residual DNA Sample Preparation Kit (Magnetic Bead Method) - Enhanced	HYS001-plus
HEK293 resDNA Quantitation Kit (PCR-Fluorescence Probe)	HYR001
E.coli resDNA Quantitation Kit (PCR-Fluorescence Probe)	HYR002
CHO resDNA Quantitation Kit (PCR-Fluorescence Probe)	HYR003
Vero resDNA Quantitation Kit (PCR-Fluorescence Probe)	HYR005
CHO resDNA Fragment Analysis and Detection Kit	HYR006
HEK293 resDNA Fragment Analysis and Detection Kit	HYR007
Human resDNA Quantitation Kit (PCR-Fluorescence Probe)	HYR013

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