Lentivirus Titer Detection Kit [HYV002]

Type: ElISA
Product Code: HYV002
Specification: 96 tests/kit
Quantification Range:3.125 pg/mL - 200 pg/mL

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Product Introduction

Product Overview

The Lentivirus Titer (HIV-1 p24) ELISA Kit employs a sandwich ELISA method to accurately, rapidly, and specifically detect the concentration of HIV-1 p24 protein in samples. It is suitable for determining p24 protein concentrations in any lentivirus based on HIV-1.

Product Performance

Validation Parameters		Validation Results
Linear Range	Quantitative Range	3.125 pg/mL - 200 pg/mL
Linear Range	R²	>0.99
Specificity		Detection results for samples including 10 ng/mL BSA, C8166 cell protein, Vero cell protein, HEK293 cell protein, HEK293T cell protein, and BHK-21 cell protein were all below the lower limit of quantification
Robustness		Experiments remain valid with ±20% variation in reaction time, ±20% variation in reaction temperature, and ±20% variation in reaction volume, meeting experimental validity standards
Precision	Intra-batch Precision	%CV < 10%
Precision	Inter-batch precision	%CV < 15%
Accuracy	Relative Error (RE)	Intra-batch %RE <±20%, inter-batch %RE < ±20%
Sensitivity	Limit of Detection (LOD)	0.861 pg/mL
Sensitivity	Lower limit of quantification (LLOQ)	3.125 pg/mL
Hook Effect		No hooking effect is observed when sample concentrations do not exceed 20 μg/mL
Stability		Key components including Micro ELISA Plate, Standard, and Detection Solution demonstrate accelerated stability at 37°C for no less than 10 days

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